Confocal leica sp5 user manual

This system has five lasers generating nine lines for fluorescence excitation 405, 458, 476, 488, 496, 514, 543, 594, 633 nm. Typing your password identifies you as a valid user for this system. The system provides the full range of scan speeds at the highest resolution. Quick guide for stepbystep protocols for the startup and closingdown of the sp5 are given at this link.

This microscope can accommodate specimens labeled with all fluorophores including ultra violet dapi. Andor dragonfly spinning disk confocal microscope users. Leicadmi6000 user manual this manual by leica microsystems gives a detailed description of the dmi6000 inverted microscope base startup and rundown procedures. The leica tcs sp5 is supplied with the latest version of the licensed las af software.

A short tutorial about the main components of a confocal laser scanning. Instructions for the leica sp5 ii laser scanning confocal microscope content. If required, switch on the mercury vapour arc lamp for detailed user guide see chapter 3. Zeiss axio observer z1 manual single or tile imaging with zen. The leica tcssp5 upright confocal laserscanning microscope is located in room 250 west. Leica sp5 confocal microscope four laser uv, argon, dpss, hene. Leica sp5 training manual september 2010 for the argon laser, adjust the slider to 2030%. If you are the last user fo the day, turn off the complete system incl. Leica sp5 ii confocal user guide school of dentistry. Choose the objective 3 in figure 1 you want to use. Leica sp5 laser scanning confocal microscope training.

The leica sp5 can capture images at image sizes ranging from 512x512 pixels to 8196x8196 pixels, z slices at 0. Leica tcs sp5 user guide leica sp5sted confocal microscope. Quick operation manual for leica sp5 confocalmultiphoton microscope. Introduction to confocal laser scanning microscopy leica. The system provides the full range of scan speeds at the highest resolution with its proven, highefficiency sp detection five channels simultaneously and optional aobs acousto optical bream splitter, the leica tcs sp5 delivers bright, noisefree. Leica sp5 inverted confocal duke light microscopy core.

To maintain information on the most current level, the description of software functions was intentionally omitted from this operating manual. Be aware, when you switch between the dry and oilwater objectives, you need to push the objective icon. Leica sp5 laser scanning confocal microscope ucsf drc. Leica tcs sp5 confocal this is a very flexible and fast confocal system build with a leica dmi 6000 cs inverted, fully automated microscope with motorized stage, condenser, objective and filter turrets.

The leica sp5 has four laser lines 405,488458, 476, 496, 514 561, and 633nm and is capable of capturing 5 channels which includes a dic brightfield detector. Rmbf leica sp5 aobs spectral confocal core facilities. Leica sp5 upright confocal, multiphoton, flim cell. Pmts photomultiplier tubes or hyds gaasp hybrid detectors. Leica tcs sp5 confocal user manual only people who have had an introduction from kees straatman are allowed to operate the system. This video shows how to configure the internal laser power wavelength etc. Leica tcs sp5 ii laser scanning confocal microscope on an inverted microscope platform. Leica dm2500 upright fluorescent microscope manuals. Log into user profile press ctrl, alt and delete simultaneously enter userid and password. Push the eye icon 2 in figure 1 to open the port for eyepieces 2. Leica tcs sp5 upright confocal laserscanning microscope. These features make the leica sp5 mp confocal a highly sensitive, flexible 2photon imaging system for thick, live tissue as well as routine confocal imaging of fixed samples. The leica tcs sp5 upright confocal laserscanning microscope is located in room 250 west. Using the uacc leica sp5 confocal page 2 how big should your image be.

Start with a laser intensity of around 15 % for mean signal intensities. Leica application suite x las x is the one software platform for all leica microscopes. Andor dragonfly spinning disk confocal microscope users manual. Ultimate optical design and stability with extreme sensitivity and superb image quality. Only people who have had an introduction from kees straatman are allowed to operate the system. The content is designed to support beginners, experienced practitioners and scientists alike in their everyday work and experiments. Leica tcs sp5 confocal laser scanning microscope user guide photometric 5 model upright dm 6000s. To learn about the leica confocal software, use the book as a tutorial. Improving image quality on the uacc leica sp5 confocal. Leica tcs sp5 user guide leica sp5 sted confocal microscope. Detection features 2 apds for confocal imaging emission separation userexchangeable beam splitting.

Researchers have the option of using three different lasers an argon, krypton, and a heliumneon laser to excite fluorescently. The leica tcs sp5 contains many functions in its user interface that reflect its wide range of potential applications. Leica microsystems sets a new standard with the integration of the leica dm6000 cfs fixed stage microscope into the leica tcs sp5 confocal platform. Instructions for the leica sp5 ii laser scanning confocal microscope. The functions not needed for a given application are disabled, however, to ensure efficiency and ease of use. Leica tcs sp5 confocal louisiana state university health. The leica sp5 confocal is a dmi 6000 model inverted microscope with a super z galvo stage for fast z sectioning and prisms for dic imaging. Log into user profile press ctrl, alt and delete simultaneously enter userid and password 5. National chiao tung university, bio photonics lab, bypraveen kumar. The confocal microscope system for highest demands in multi user facilities. View online or download leica sp5 confocal user manual. Leica dm2500 upright fluorescent microscope manuals cell. Leica sp5 confocal user manual date of first issue. It integrates confocal, widefield, stereo, superresolution, and lightsheet instruments from leica microsystems.

These features make the leica sp5 mpconfocal a highly sensitive, flexible 2photon imaging system for thick, live tissue as well as routine confocal imaging of fixed samples. Leica tcs sp5 confocal laser scanning microscope user guide. Leica sp5 general training manual sept 2010 robarts. The leica sp5 does not use emission filters to define the wavelengths of emission collected, but. It is recommended setting up a separate user id for each user set up by the system administrator.

Confocal microscopy facility schepens eye research. The olympus dsu disk scanning unit offers leadingedge spinning disk confocal technology. How to use leica confocal microscope sp5 external laser part1. Leica sp5c spectral confocal laser scanning microscope. Sp5 confocal microscope user manual, helene javot updated by mamta srivastava. How to use leica confocal microscope sp5 external laser. The leica sp5 laser scanning confocal system is used for advanced multichannel fluorescence and livecell applications. On the touchscreen display panel of the leica dmi8 microscope, push magnification icon 1 in figure 1 to open the control panel.

Checkin and start up set up acquistion parameters optimize acquistion parameters acquire a zstack sequential scan check out appendix a printable pdf checkin and start up. Tirf, srrfstream, point localization superresolution. Pcic sp5 confocal microscope user manual, helene javot updated by mamta srivastava 2112017, page 116 pcic plant cell imaging center bti, room 104b confocal leica sp5 user manual startup procedure. Note, just because the instrument has these capabilities, does not mean that you need.

Leica tcssp5 upright confocal laserscanning microscope. Leica sp5 inverted confocal duke light microscopy core facility. Checkin and start up set up acquistion parameters optimize acquistion parameters acquire a zstack sequential scan check out appendix. Leica sp5 inverted confocal manual duke light microscopy.

Sequential scanning using the leica tcs sp5 confocal laser scanning microscope this supplemental manual is the second section of a three part leica tcs sp5 user guide edited by donald pottle schepens 149 w dwp mar. The leica tcs sp5 confocal fully covers a broad range of requirements in confocal and multiphoton imaging with excellent overall performance. The system was designed for confocal scanning laser scanning images of fluorescence marked living and fixed specimens as well as for quantitative. Laser point scan confocal with standard and highspeed resonance scanning. Two of these detectors are very sensitive hyd sp gaaspdetectors. Science lab, the knowledge portal of leica microsystems, offers scientific research and teaching material on the subjects of microscopy. The new leica tcs sted cw confocal microscope is the most straightforward way to user manual.

Designed by olympus, the dsu disk contains a pattern of slits that creates a virtual pinhole as the disk spins at 3,000 rpm. Leica dmi6000cs inverted live cell chamber ludin cube metal halide fluorescence source motorized scanning stage tandem scanners select at software initialization conventional scanner resonant scanner 8 000 hz lasers. Acute and chronic live cell and living tissue imaging. Note, just because the instrument has these capabilities, does not. Quick operation manual for leica sp5 confocalmultiphoton. Leica application suite x las x is the easy to use software platform for advanced life science research on leica microsystems confocal, widefield. Gently tilt back the arm that holds the transmitted light illumination. The following is the details for confocal platform as well as two microscopes.

Helene javot, sp5 confocal microscope user manual, updated by mamta srivastava 2 022012, page 1 15 pcic plant cell imaging center bti, room 104b confocal leica sp5 user manual startup procedure. Leica tcs sp5 ii the only broadband confocal technical documentation living up to life. Confocal and multiphoton microscopy core school of dentistry. Hexagonal packing of nuclei red by microtubules green in coeonocytic female gametophyte of ginkgo biloba. The leica sp5 is a pointscan confocal microscope with laser lines at 405, 458, 476, 488, 496, 514, 543, 594, and 633nm. Remove the dust cover from the microscope turn on the mercury lamp a if you need to see the fluorescence through the eye pieces. Remove the dust cover from the microscope turn on the mercury lamp if you need to see the fluorescence through the eye pieces. The microscope is an upright dm6000 and has a motorized stage. The system was designed for confocal recor ding laser. If there is a gap, turn off the system lasers, mercury lamp, turn off the software but leave computer and hardware running. Turning on the confocal system instructions are posted in the room 1. In addition to confocal imaging of fixed tissue samples and cells, this system is additionally equipped for. An introduction from your bosssupervisor is not enough the system has 5 detectors. Neurobiological research has a long history not only in measurements of single cells, but also of thick brain slices.

The sp5 is also equipped with an optional 6position motorized objective turret that holds a standard set of air and oil objectives for confocal imaging of samples. Usermanual for leica sp8 confocal universitetet i bergen. The system is equipped with an inverted microscope encased with an incubator for livecell imaging. Leica am total internal reflection fluorescence manual. Leica application suite x microscope software youtube. Jan 04, 2018 this video shows how to configure the internal laser power wavelength etc. Leica sp5 scanning confocal cdb equipment resource. A better understanding of your confocal system will allow you to modify later on scan format and speed when appropriate. Training manual leica tcs sp5 confocal microscope september 2010. The book usually introduces a set of steps to accomplish a particular task with a distinctive heading. This leica sp2 platform is equipped with two leica confocal microscopes. Turning on the system 1turn on the fluorescent light source number 1.

This manual is the first section of a three part leica tcs sp5 user guide. Leica tcs sp5the newest generation of leica confocal microscopes the zmb owns an inverted leica tcs, a tsc sp2 confocal system with upright and inverted microscope stand and. From basic documentation to advanced life science research las x. A high sensitivity, inverted, pointscanning confocal system. Leica application and technology support group, life science division exton, pa 19341, usa leica tcs sp5 lasaf new user guide. Microscope leica sp5 confocal user manual 65 pages microscope leica sp8 training manual. Here more realistic results can be achieved due to the intact network. Microscopists utilize the scanning stage for setting up multiple zstacks with the marknfind option and for stitching numerous fields into one large image. The leica tcs is a universal laser scanning confocal microscope system for the biomedical and materials research environment. Dmi 4000 cs scanner is mo from confocal ob and eyepiece torized.

Manual leica tcs sp ii university of massachusetts. Checkin and start up set up acquistion parameters optimize acquistion parameters acquire a zstack sequential scan check out appendix checkin and start up. If there is a gap, turn off the system lasers, mercury lamp, turn off the software but leave. Leica sp5 inverted confocal manual duke light microscopy core. Instructions for the leica sp5 ii laser scanning confocal. Activate the laser lines according to your fluorochromes. The confocal microscope system for highest demands in multiuser facilities. Overview highspeed and highsensitivity multimodal confocal imaging with 2 pinhole sizes 25um and 40um four laser lines.